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A researcher at the University of Amsterdam has come up with a way of seeing a three dimensional image of transparent materials through a microscope.
Rajesh S. Pillai used a laser that emits extremely short pulses of infrared light. These pulses cause molecules to respond as if they have been hit very hard, as a result of which they emit light of exactly three times the frequency of the incoming light. By measuring this light, it is possible to build up a three dimensional image. The pulses of infrared light are so short that the molecules are not permanently damaged.
Pillai has used the technique to examine the microstructure of food and to visualise the formation and breakdown of lipid droplets in cells, opening up a means for directly observing the process of fat storage in humans.
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